Customer Success Story

Using The Cell Growth Quantifier (CGQ) For Phototrophic Cultures

Customer

Prof. Dr. Ilka Maria Axmann (Heinrich-Heine-Universität Düsseldorf, Germany)

Background

The unicellular cyanobacterium Synechococcus elongatus PCC 7942 (S. elongatus), growing optimally at 33 °C and light intensities of 120 µmol/m2 · s, thrives in freshwater ponds and is commonly used as a phototrophic model organism (Kuan et al. 2015). Here, we characterized the growth of S. elongatus in a CO2 incubator shaker under constant light conditions.

 

Fig. 1 Growth curves of S. elongatus in BG-11 medium as measured by the Cell Growth Quantifier (CGQ). Online backscatter intensity growth curves were compared to manual offline CDW measurement. Cultivation conditions: 30 °C, 0,5 % CO2, 75 % humidity, 150 rpm, 80 µmol/m2 · s light intensity with an Infors Multitron incubator shaker.

Testimonial

“For the first time, we were able to generate high-quality growth curves of photosynthetic S. elongatus without necessarily relying on classical manual measurements like OD750 or cell dry weight, especially when it comes to high cell densities during our very long cultivation times of 16 days and more. Thus, we don’t face the omnipotent risk of contamination anymore. This greatly boosts our experimental shake flask throughput. My team and I really appreciated the performance of the CGQ and we’re looking forward to using it in our daily lab routine!”

Prof. Dr. Ilka Maria Axmann (University of Düsseldorf)

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