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What Our Customers Are Saying

"With the DOTS platform I can combine sensors and actuators in a simple way to perform completely new applications like biomass-based feeding in shake flasks. My shake flasks can now operate similar to small-scale bioreactors, keeping the ease and cost-effectiveness of flasks but adding the sensor and control options of bioreactors. This is truly revolutionary for me and my experiments."

-Christoph Kutzner (Badische Peptide & Proteine, Heidelberg)

 

Applications

Biomass Monitoring in Shake Flasks

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Online biomass monitoring via backscattered light readings can be applied in shake flasks (CGQ) and in multiple, parallel experiments. High data density helps you to detect relevant growth events and enables actionable insights into your bioprocess.

Biomass-based Feeding in Shake Flasks

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Use the interconnectivity of sensors (CGQ) and actuators (LIS) within the DOTS Software to enable feedback controlled feeding in your shake flask experiments. Set up your injection strategy based on your biomass threshold or growth rate and start feeding based on the specific needs of your culture.

Biomass-based Feeding

Liquid Feeding in Shake Flasks

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The Liquid Injection System (LIS) enables automated and non-invasive feeding in shake flasks. Simply assemble the components, define experiment parameters, fill the cartridge with the feeding liquid, and start feeding. Control LIS Drives wirelessly by using the DOTS Software for a fully automated bioprocess in shake flasks.

LIS

pH & DO Monitoring in Flow Loops

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Flow Cells containing sensor spots for pH and dissolved oxygen (DO) allow for a flexible monitoring of these parameters in various vessels and locations, including hard-to-reach places. Monitor pH and DO in real-time to ensure the well-being of your cell culture.

Biomass Monitoring in Bioreactors

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Online biomass monitoring via backscattered light readings can be applied in bioreactors (CGQ-BioR) and in multiple, parallel experiments. High data density helps you to detect relevant growth events and enables actionable insights into your bioprocess.

Monitor Environmental Data

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Collecting environmental data, like temperature or shaking speed, for your shake flask experiments allows you to dive deeper into your culture’s conditions. DOTS sensors monitor environmental parameters in addition to their sample-centric measurements avoiding the need of additional sensors.

 

 

 

Powered By DOTS Software

DOTS Software is your tool for easy handling of sensors, actuators, and bioprocess data, offering total control, from calibration to data collection.
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The Future of DOTS

Bioprocessing is getting more and more complex and we still see too many un-monitored processes or manual sampling. Our vision is a sensor platform that will truly allow you to monitor every point of interest when and where it matters to connect the DOTS in bioprocessing.

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Multiparameter Sensors

Our future multiparameter sensors will measure a broad variety of conditions. Simply mount the sensor at your point of interest and measure your parameters wirelessly, regardless of vessel type or application.

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IoT Infrastructure

We are building a wireless sensing and IoT infrastructure to allow devices to be connected, sensed, and controlled remotely, ensuring the best sensing experience for you.

integrations

Third Party Integration

We will expand the ability to integrate with existing laboratory bioprocess equipment and data management systems for ultimate flexibility.

Specifications

DOTS offers the simplest sensor handling in bioprocessing for a broad variety of parameters, vessel types, and applications.

Parameters

  • Biomass & growth rate
  • pH
  • Dissolved Oxygen (DO)
  • Temperature & shaking speed
  • Time-based & parameter-based feeding

Vessels

  • Shake flasks of all common sizes
  • Single-use shake flasks
  • Serum bottles, Schott bottles
  • Bioreactors from benchtop to large scale production
  • Customized bioreactors for your specific application (e.g., phototrophic bags or tubes)
  • Flow loops (e.g., feeding, sampling, or harvesting flow loops in bioreactors)

Applications

  • Strain development, screening, and characterization

  • Bioprocess optimization

  • Media optimization

  • Growth characterization (including toxicity tests, substrate-/product-inhibitions, and oxygen-limitations)

  • Timing of workflows (such as inoculation, induction, sampling, feeding, or harvesting)

  • Bioreactor-like experiments on shake flask level (e.g., automatized induction, biomass-based fed-batch processes)

Resources

Compatibility

DOTS offers the simplest sensor handling in bioprocessing for a broad variety of parameters, vessel types, and applications.

Parameters

  • Biomass & growth rate
  • pH
  • Dissolved Oxygen (DO)
  • Temperature & shaking speed
  • Time-based & parameter-based feeding

Vessels

  • Shake flasks of all common sizes
  • Single-use shake flasks
  • Serum bottles, Schott bottles
  • Bioreactors from benchtop to large scale production
  • Customized bioreactors for your specific application (e.g., phototrophic bags or tubes)
  • Flow loops (e.g., feeding, sampling, or harvesting flow loops in bioreactors)

Applications

  • Strain development, screening, and characterization

  • Bioprocess optimization

  • Media optimization

  • Growth characterization (including toxicity tests, substrate-/product-inhibitions, and oxygen-limitations)

  • Timing of workflows (such as inoculation, induction, sampling, feeding, or harvesting)

  • Bioreactor-like experiments on shake flask level (e.g., automatized induction, biomass-based fed-batch processes)

Resources

Resources

From Estimation To High-Resolution Growth Curves

Cell Growth Quantifier (CGQ)
Success Growth Graph

Customer Success Stories

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“Incorporating SBI’s pH and DO flow cells into our system removed the need for manual sampling, saving us time, reducing the risk of contamination, and providing information on how the cells are growing even when we are not in the lab. With availability of this more detailed view of our culture, we can make informed improvements to our cell expansion process.”

-Kitana Manivone Kaiphanliam (Washington State University)
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Want To Connect The DOTS In Your Bioprocessing?

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