Overview

Open a whole new world of bioprocess development when you use LIS to perform automatic feeding in shake flasks. Simply assemble the components, define the experiment parameters, fill the cartridge with the feeding liquid, and start feeding.

  • Automated feeding of liquids into shake flask cultures
  • Flexible feeding profiles: Delay, single shot, multi shot, continuous, or exponential
  • New Application! Combine with CGQ for biomass-based feeding in shake flasks
  • Control and monitor your feeding experiments wirelessly with the DOTS Software
  • For a broad variety of applications (e.g., Fed-Batch, pH control or automated inoculation)
  • Control and monitor your feeding experiments wirelessly with the DOTS Software

What Our Customers Are Saying

"Overall, the LIS system is easy to assemble, reduces manual work significantly, and allows us to test multiple feeding profiles, including linear and exponential feeding.”

-Prof. Dr. -Ing. Frank Eiden (Westphalian University of Applied Science)
WHS
"LIS- and CGQ-system turns nights and weekends into productive times for the preparation of optimal inocula and for the first time leverages the early stages of culture expansion to a level of bioprocess monitoring and control that has until today only been accessible in later stage stirred tank bioreactors.”

-FermFactory (RWTH Aachen University)
FermFactory (RWTH Aachen University)
With the DOTS platform I can combine sensors and actuators in a simple way to perform completely new applications like biomass-based feeding in shake flasks. My shake flasks can now operate similar to small-scale bioreactors, keeping the ease and cost-effectiveness of flasks but adding the sensor and control options of bioreactors. This is truly revolutionary for me and my experiments.

-Christoph Kutzner (Badische Peptide & Proteine, Heidelberg)
Badische Peptide & Proteine-1

How It Works

Automation enables standard use of complex feeding schedules.

Liquid Injection System (LIS)

In order to dispense a defined amount of liquid from the cartridge, the drive pumps air through a sterile filter into the cartridge according to the feeding profile. 

Liquid Injection System (LIS)

After the dispense step, the drive rebuilds negative pressure to keep the remaining liquid in the cartridge.

 

Hardware & Software Components

LIS Drive

LIS Drive

The drive is a programmable miniature pump that controls when and how much liquid is fed from the cartridge into the flask.

LIS Cartridge

LIS Cartridge

The cartridge is a  pre-sterilized, ready-to-use container that fits on top of the shake flask. It can be filled with up to 25 mL of any type of liquid and acts as a reservoir during the feeding experiment.

DOTS Software

SBI-Icon-dots-software-computer-2

The DOTS Software allows you to wirelessly control and monitor your feeding experiments. Create individual feeding profiles, define feeding parameters, and monitor the expensed liquid volume over time. By combining other DOTS integrated sensors, parameter-based feeding can be realized.

Specifications

Flask

Compatible Laboratory Infrastructure

The LIS is designed to fit any 38 mm straight neck Erlenmeyer shake flask.

  • Any shake flask size ranging from 100 ml to 5000 ml

  • Flasks with or without baffles

  • Glass and single-use plastic flasks

  • Spring clip and Sticky Stuff mounts

LIS has been successfully tested with a broad variety of liquids. Our team of application scientists will work with you to ensure that the liquid and concentration needed for your particular application can be used with our feeding system. 

  • Sugars

  • Alcohols

  • Glycerol

  • Acids, bases, and inductors (e.g., IPTG)

  • Antifoam

 

Vessel Types
Flask

Compatible Laboratory Infrastructure

The LIS is designed to fit any 38 mm straight neck Erlenmeyer shake flask.

  • Any shake flask size ranging from 100 ml to 5000 ml

  • Flasks with or without baffles

  • Glass and single-use plastic flasks

  • Spring clip and Sticky Stuff mounts

Liquids

LIS has been successfully tested with a broad variety of liquids. Our team of application scientists will work with you to ensure that the liquid and concentration needed for your particular application can be used with our feeding system. 

  • Sugars

  • Alcohols

  • Glycerol

  • Acids, bases, and inductors (e.g., IPTG)

  • Antifoam

 

Resources

Customer Success Stories

Liquid Injection System (LIS)

How to optimize bioreactor inoculum in shake flasks?

This application note illustrates the use of LIS in conjunction with the yeast S. cerevisiae to optimize bioreactor inoculum in shake flasks. After a period of preconditioning, the LIS automatically injects different seed biomass concentrations into a shake flask containing fresh YEPD. Cell growth is monitored in real-time to assess the reproducibility of environmental and growth phase conditions.

Want To Connect The DOTS In Your Bioprocessing?