Use the interconnectivity of sensors (CGQ) and actuators (LIS) within the new DOTS Software to enable feedback controlled feeding in your shake flask experiments. Set up your injection strategy based on your biomass threshold or growth rate and start feeding based on the specific needs of your culture.
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Contents
What Our Customers Are Saying
"Overall, the LIS system is easy to assemble, reduces manual work significantly, and allows us to test multiple feeding profiles, including linear and exponential feeding.”
-Prof. Dr. -Ing. Frank Eiden (Westphalian University of Applied Science)
-Prof. Dr. -Ing. Frank Eiden (Westphalian University of Applied Science)
"LIS- and CGQ-system turns nights and weekends into productive times for the preparation of optimal inocula and for the first time leverages the early stages of culture expansion to a level of bioprocess monitoring and control that has until today only been accessible in later stage stirred tank bioreactors.”
-FermFactory (RWTH Aachen University)
-FermFactory (RWTH Aachen University)
"With the DOTS platform I can combine sensors and actuators in a simple way to perform completely new applications like biomass-based feeding in shake flasks. My shake flasks can now operate similar to small-scale bioreactors, keeping the ease and cost-effectiveness of flasks but adding the sensor and control options of bioreactors. This is truly revolutionary for me and my experiments."
-Christoph Kutzner (Badische Peptide & Proteine, Heidelberg)
-Christoph Kutzner (Badische Peptide & Proteine, Heidelberg)
"By using the LIS, I can now continuously feed glucose in shake flasks. This improves the transferability of results from shake flask experiments to bioreactor runs."
-Dr. Kambiz Morabbi Heravi (Project Manager, Strain Engineering, Clariant)
-Dr. Kambiz Morabbi Heravi (Project Manager, Strain Engineering, Clariant)
How It Works
Automation enables standard use of complex feeding schedules.
Hardware & Software Components
LIS Drive
LIS Cartridge
DOTS Software
Specifications
Compatible Laboratory Infrastructure
The LIS is designed to fit any 38 mm straight neck Erlenmeyer shake flask.
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Any shake flask size ranging from 100 mL to 5000 mL
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Flasks with or without baffles
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Glass and single-use plastic flasks
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Spring clip and Sticky Stuff mounts
LIS has been successfully tested with a broad variety of liquids. Our team of application scientists will work with you to ensure that the liquid and concentration needed for your particular application can be used with our feeding system.
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Sugars
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Alcohols
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Glycerol
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Acids, bases, and inductors (e.g., IPTG)
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Antifoam
Vessel Types
Compatible Laboratory Infrastructure
The LIS is designed to fit any 38 mm straight neck Erlenmeyer shake flask.
-
Any shake flask size ranging from 100 mL to 5000 mL
-
Flasks with or without baffles
-
Glass and single-use plastic flasks
-
Spring clip and Sticky Stuff mounts
Liquids
LIS has been successfully tested with a broad variety of liquids. Our team of application scientists will work with you to ensure that the liquid and concentration needed for your particular application can be used with our feeding system.
-
Sugars
-
Alcohols
-
Glycerol
-
Acids, bases, and inductors (e.g., IPTG)
-
Antifoam
Resources
Customer Success Stories
With LIS, typical bioreactor processes, like fed-batch cultivations, can be recreated in shake flasks.
Fed-batch cultures support the growth of S. cerevisiae on potato waste because the concentration of growth inhibitors in the medium can be controlled. With the Liquid Injection System (LIS), these process conditions could be imitated on a shake flask level.
LIS WIDENS THE APPLICATION AREA FOR SHAKE FLASKS, E.G., TO DETERMINE THE BEST PRODUCTION STRAIN.
The batch condition has a great impact on the cell's metabolism which overshadows the strain's characteristic protein production behavior. A LIS facilitated fed-batch process showed a significant increase (30%) in active protein yields, compared to a batch process and is better suited to screen for the best production strain.
