Overview

Monitor biomass and growth rates in your bioreactors with high-resolution growth curves using the CGQ BioR. Optical biomass measurements are based on scattered light detection without the use of integrated sensors. Simply mount the sensor plate to the outside of your reactor and monitor the biomass through the vessel wall. Follow the growth of your cultures in real time to gain actionable insights into your strain’s growth behavior.

  • Save time with automated, online, non-invasive biomass monitoring in up to 64 bioreactors in parallel
  • 2 measurement modes for high and low biomass concentrations
  • No cleaning or sterilization required
  • Free up reactor port space
  • Flexible integration of biomass data into other software (e.g., analog, OPC…)
  • Gain actionable insights with high resolution growth curves
  • Powerful software for experiment control and advanced data analytics

What Our Customers Are Saying

"The automated CGQ BioR system provides precise growth curves with a very dense sampling interval, freeing my time for tasks with higher added value.”

-Prof. Dr. -Ing. Thomas Walther (TU Dresden)
TU Dresden

How it Works

The CGQ’s technique for noninvasive cell density monitoring is based on the principle of light scattering.

Using an array of LEDs and photodiodes, the sensor emits light through the wall of the vessel and measures the amount of light that is scattered back. The more cells that are in the reactor, the more light that is scattered back. This backscatter signal can be correlated with offline cell density data, such as OD600 or cell dry weight (CDW).

CGQ Sensing
Architecture

CGQ Sensing Architecture
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The sensor of the CGQ BioR contains two LEDs and a photodiode detector. The two LEDs with different wavelengths are used to allow for precise measurements at low and high cell densities.

Biomass Monitoring by Backscattered Light

Biomass Monitoring by Backscattered Light
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Light is emitted from the LEDs into the culture medium. While most photons go straight through the broth, some are scattered by the cells and return to the photodiode, which measures the scattered light intensity. The higher the cell density, the more light is scattered back to the photodiode.

Hardware & Software Components

CGQ Sensor

CGQ Sensor
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Each sensor is positioned outside of the bioreactor, measuring the biomass through the vessel wall.

Base Station

Base Station
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Several sensors can be connected to a single base station. The base station bundles the data from all monitored flasks and sends it to the software.

CGQuant Software

CGQuant Software
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The software then analyzes the biomass signals and presents it as a real-time growth curve. Datasets can be easily exported for further data analysis. 

Specifications

Compatible Laboratory Infrastructure

The CGQ BioR is compatible with a variety of bioreactors. Different sized attachment straps provide maximum flexibility, allowing the same sensor to be used with different bioreactor sizes and manufacturers.

For different reactor types:

  • Single-jacket
  • Double-jacket

For different reactor sizes:

  • Mini bioreactor systems
  • Bench top bioreactors
  • Production scale

Compatible Organisms

While application requirement can vary, the CGQ BioR has proven successful in monitoring used for many different types of cultures including:

Bacteria

  • Escherichia coli
  • Corynebacterium glutamicum
  • Bacillus subtilis
  • Pseudomonas putida
  • Pseudomonas taiwanensis
  • Gluconobacter oxydans
  • Lactobacillus plantarum
  • Vibrio natriegens
  • Vibrio cholerae
  • Staphylococcus aureus
  • Klebsiella pneumoniae
  • Actinobacillus pleuropneumoniae
  • Chromobacterium violaceum
  • Blautia producta
  • Hungtatella hathewayi
  • Prevotella copri

Yeast

  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Pichia pastoris
  • Yarrowia lipolytica
  • Kluyveromyces lactis
  • Hansenula polymorpha
  • Ustilago maydis

Filamentous organisms

  • Aspergillus fumigatus
  • Aspergillus nidulans
  • Aspergillus niger
  • Streptomyces acidiscabies
  • Streptomyces venezuelae
  • Trichoderma reesei

Archaea

  • Haloferax volcanii

  • Sulfolobus acidocaldarius

Anaerobic organisms

  • Acetobacterium woodii

  • Clostridium aectobutylicum

  • Clostridium ljungdahlii

  • Clostridium difficile

Phototrophic organisms

  • Chlorella vulgaris

  • Scenedesmus obliquus

  • Synechococcus elongatus

  • Nicotiana tabacum BY-2 (plant cells)

Resources

Compatibility

Compatible Laboratory Infrastructure

The CGQ BioR is compatible with a variety of bioreactors. Different sized attachment straps provide maximum flexibility, allowing the same sensor to be used with different bioreactor sizes and manufacturers.

For different reactor types:

  • Single-jacket
  • Double-jacket

For different reactor sizes:

  • Mini bioreactor systems
  • Bench top bioreactors
  • Production scale
Organisms

Compatible Organisms

While application requirement can vary, the CGQ BioR has proven successful in monitoring used for many different types of cultures including:

Bacteria

  • Escherichia coli
  • Corynebacterium glutamicum
  • Bacillus subtilis
  • Pseudomonas putida
  • Pseudomonas taiwanensis
  • Gluconobacter oxydans
  • Lactobacillus plantarum
  • Vibrio natriegens
  • Vibrio cholerae
  • Staphylococcus aureus
  • Klebsiella pneumoniae
  • Actinobacillus pleuropneumoniae
  • Chromobacterium violaceum
  • Blautia producta
  • Hungtatella hathewayi
  • Prevotella copri

Yeast

  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Pichia pastoris
  • Yarrowia lipolytica
  • Kluyveromyces lactis
  • Hansenula polymorpha
  • Ustilago maydis

Filamentous organisms

  • Aspergillus fumigatus
  • Aspergillus nidulans
  • Aspergillus niger
  • Streptomyces acidiscabies
  • Streptomyces venezuelae
  • Trichoderma reesei

Archaea

  • Haloferax volcanii

  • Sulfolobus acidocaldarius

Anaerobic organisms

  • Acetobacterium woodii

  • Clostridium aectobutylicum

  • Clostridium ljungdahlii

  • Clostridium difficile

Phototrophic organisms

  • Chlorella vulgaris

  • Scenedesmus obliquus

  • Synechococcus elongatus

  • Nicotiana tabacum BY-2 (plant cells)

Resources

Resources

From Estimation to Information

Why CGQ for your Bioreactors?

Manual sampling-based data is often not sufficient to fully understand the bioprocess. Offline sampling is complex and time consuming, resulting in lower measurement frequency with most pulls being at the start or the end of the experiment. This means that critical information from your growth phases are being overlooked, and could have a detrimental impact on your final product. Automated online measurements, on the other hand, never miss a moment. With a high resolution growth curve, you can detect bioprocess changes in real time.

Cell Growth Quantifier for Bioreactors (CGQ BioR)
Cell Growth Quantifier for Bioreactors (CGQ BioR)

Customer Success Stories

Cell Growth Quantifier for Bioreactors (CGQ BioR)

Using The CGQ Bior To Monitor K. Marxianus During The Production Of Ethyl Acetate

Yeasts ferment to produce ethyl acetate (EA), a widely used solvent. In this experiment, Kluyveromyces marxianus was cultivated in a synthetic medium and the CGQ BioR was used to calculate biomass-specific EA synthesis rates to identify optimal production stages.

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