Monitor biomass and growth rates in your cultivation vessels with high resolution using the CGQ. Optical biomass measurements are based on scattered light detection without the use of integrated sensors. Simply install the sensor plate under the shake flask and monitor the biomass through the vessel wall. Follow the growth of your cultures in real time to gain actionable insights into your strain’s growth behavior.
Using an array of LEDs and photodiodes, the CGQ sensor emits light into the vessel and measures the amount of light that is scattered back. The more cells that are in the flask, the more light is scattered back. This backscatter signal can be correlated with offline cell density data, such as OD600 or cell dry weight (CDW).
The sensor plate of the CGQ contains an LED light source and a photodiode detector. The CGQ’s innovative measuring method treats the signal fluctuations from the moving bulk liquid due to shaking as a valuable information source rather than noise.
Light is emitted from the LED into the culture medium. While most photons go straight through the broth, some are scattered by the cells and return to the photodiode, which measures the scattered light intensity. The higher the cell density, the more light is scattered back to the photodiode.
Each sensor plate is positioned under the cultivation vessel and measures the biomass non-invasively through the vessel wall. If needed, each shake flask is darkened with a cover to ensure precise measurements.
Several sensor plates can be connected to a single base station. The base station bundles the data from all monitored flasks and sends it to the software.
The software then analyzes the biomass signals and presents it as a real-time growth curve. Datasets can be easily exported.
The CGQ is compatible with a variety of vessel types. An available adapter system provides maximum flexibility, allowing the same sensor plate to be used with different shake flask sizes.
Any shake flask size ranging from 100mL to 5000mL
Flasks with or without baffles
Glass and single-use flasks
Spring clip and sticky plate mounts
Escherichia coli
Corynebacterium glutamicum
Bacillus subtilis
Pseudomonas putida
Pseudomonas taiwanensis
Gluconobacter oxydans
Lactobacillus plantarum
Vibrio natriegens
Vibrio cholerae
Staphylococcus aureus
Klebsiella pneumoniae
Actinobacillus pleuropneumoniae
Chromobacterium violaceum
Blautia producta
Hungtatella hathewayi
Prevotella copri
Saccharomyces cerevisiae
Schizosaccharomyces pombe
Pichia pastoris
Yarrowia lipolytica
Kluyveromyces lactis
Hansenula polymorpha
Ustilago maydis
Aspergillus fumigatus
Aspergillus nidulans
Aspergillus niger
Streptomyces acidiscabies
Streptomyces venezuelae
Trichoderma reesei
Haloferax volcanii
Sulfolobus acidocaldarius
Acetobacterium woodii
Clostridium aectobutylicum
Clostridium ljungdahlii
Clostridium difficile
Chlorella vulgaris
Scenedesmus obliquus
Synechococcus elongatus
Nicotiana tabacum BY-2 (plant cells)
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